Name: Assoc.Prof.Dr. Benjamas Cheirsilp



Degree:          Ph.D. (Biotechnology Engineering), Osaka University, Japan

                        M.Eng. (Biotechnology Engineering), Osaka University, Japan

                        B.Eng. (Chemical Engineering), Tohoku University, Japan


Field of interest : Bioprocess Engineering, Enzyme Technology, Fermentation


Current researches:

Development of fermentation and enzyme production process

Biobutanol production

Oleaginous microorganism cultivation for biooil production

Mixed culture for bioproduct

Biodiesel production



Shibasaki-Kitakawa, N., Cheirsilp, B., Iwamura, K., Kushibiki, M., Kitakawa, A. and Yonemoto, T. (1998) Kinetic model for oligosaccharide hydrolysis using suspended and immobilized enzymes. Biochem. Eng. J. 1(3): 201-209

Cheirsilp, B., Shimizu, H. and Shioya, S. (2001) Modelling and optimization of environmental conditions for kefiran production by Lactobacillus kefiranofaciens. Appl. Microbiol. Biotechnol. 57:639-646. (Included in docter thesis)

Cheirsilp, B., Shimizu, H. and Shioya, S. (2003) Enhanced kefiran production of Lactobacillus kefiranofaciens by mixed culture with Saccharomyces cerevisiae. J. Biotechnol. 100(1): 43-53. (Included in docter thesis)

Cheirsilp, B., Shoji, H., Shimizu, H. and Shioya, S. (2003) Interactions between Lactobacillus kefiranofaciens and Saccharomyces cerevisiae in mixed culture of kefiran production. J. Biosci. Bioeng. 96 (3): 279-284. (Included in docter thesis)

Cheirsilp, B. (2006) Simulation of kefiran production of Lactobacillus kefiranofaciens JCM6985 in fed-batch reactor. Songklanakarin J. Sci. Technol. 28(5): 1059-1069.

Cheirsilp, B., Shimizu, H. and Shioya, S. (2007) Kinetic modeling of kefiran production in mixed culture of Lactobacillus kefiranofaciens and Saccharomyces cerevisiae. Process Biochem. 42: 570-579.

Cheirsilp, B., Kaewthong, W. and H-Kittikun, A. (2007) Kinetic study of glycerolysis of palm olein for monoacylglycerol production by immobilized lipase. Biochem. Eng. J. 35(1): 71-80.

Cheirsilp, B. and H-Kittikun, A. (2007) A mathematical model approach to a glycerolysis reaction for monoacylglycerol production. WIT Transactions on Modelling and Simulation 46: 225-232.

Yeesang, C., Chanthachum, S. and Cheirsilp, B. (2008) Sago starch as a low-cost carbon source for exopolysaccharide production by Lactobacillus kefiranofaciens. World J. Microbiol. Biotechnol. 24(7): 1195-1201.

Cheirsilp, B. and Umsakul, K. (2008) Processing of banana-based wine product using pectinase and α-amylase. J. Food Process Eng. 31: 78-90.

H-Kittikun, A., Kaewthong, W. and Cheirsilp, B. (2008) Continuous production of monoacylglycerols from palm olein in packed-bed reactor with immobilized Lipase PS. Biochem. Eng. J. 40: 116-120.

Cheirsilp, B., H-Kittikun, A. and Limkatanyu, S. (2008) Impact of transesterification mechanisms on the kinetic modeling of biodiesel production by immobilized lipase. Biochem. Eng. J. 42: 261–269.

Kitcha, S., Maneerat, S. and Cheirsilp, B. (2008) Cyclodextrin glycosyltransferase from a newly isolated alkalophilic Bacillus sp. C26. Songklanakarin J. Sci. Technol. 30(6): 723-728.

Cheirsilp, B., Jeamjounkhaw, P. and H-Kittikun, A. (2009) Optimizing an alginate immobilized lipase for monoacylglycerol production by the glycerolysis reaction. J. Mol. Catal. B: Enzym. 59: 206-211.

Tran, H.T.M., Cheirsilp, B., Hodgson, B. and Umsakul, K. (2010) Potential use of Bacillus subtilis in a co-culture with Clostridium butylicum for acetone–butanol–ethanol production from cassava starch. Biochem. Eng. J. 48: 260–267.

Cheirsilp, B., Kitcha, S. and Maneerat, S. (2010) Kinetic characteristics of beta-cyclodextrin production by cyclodextrin glycosyltransferase from newly isolated Bacillus sp. C26. Electron. J. Biotechnol. 13(4) Article Number: 6

Tongboriboon, K., Cheirsilp, B., H-Kittikun, A. (2010) Mixed lipases for efficient enzymatic synthesis of biodiesel from used palm oil and ethanol in a solvent-free system. J. Mol. Catal. B: Enzym. 67 (1-2): 52-59.

Saenge, C., Cheirsilp, B., Suksaroge, T.T., Bourtoom, T. (2011) Potential use of oleaginous red yeast Rhodotorula glutinis for the bioconversion of crude glycerol from biodiesel plant to lipids and carotenoids. Process Biochem. 46(1): 210-218.

Yeesang, C., Cheirsilp, B. (2011) Effect of nitrogen, salt, and iron content in the growth medium and light intensity on lipid production by microalgae isolated from freshwater sources in Thailand. Bioresour. Technol. 102(3): 3034-3040.

Saenge, C., Cheirsilp, B., Suksaroge T., Bourtoom, T. (2011) Efficient concomitant production of lipids and carotenoids by oleaginous red yeast Rhodotorula glutinis cultured in palm oil mill effluent and application of lipids for biodiesel production. Biotechnol. Bioprocess Eng. 16(1): 23-33.

Sri, R.M., Cheirsilp, B., Kitcha, S. (2011) Effect of substrate concentration and temperature on the kinetics and thermal stability of cyclodextrin glycosyltransferase for the production of -cyclodextrin: Experimental results vs. mathematical model. Process Biochem. 46(7): 1399–1404.

Cheirsilp, B., Suwannarat, W., Niyomdecha, R. (2011) Mixed culture of oleaginous yeast Rhodotorula glutinis and microalga Chlorella vulgaris for lipid production from industrial wastes and its use as biodiesel feedstock. New Biotechnol. 28 (4):362-368.

Cheirsilp, B., Radchabut, S. (2011) Use of whey lactose from dairy industry for economical kefiran production by Lactobacillus kefiranofaciens in mixed cultures with yeasts. New Biotechnol. 28 (6):574-580.

Suphatharaprateep, W., Cheirsilp, B., Jongjareonrak, A. (2011) Production and properties of two collagenases from bacteria and their application for collagen extraction. New Biotechnol. 28 (6): 649-655.

Noomtim, P., Cheirsilp, B. (2011) Production of butanol from palm empty fruit bunches hydrolyzate by Clostridium acetobutylicum. Energy Procedia 9: 140–146.

Kitcha, S., Cheirsilp, B. (2011) Screening of oleaginous yeasts and optimization for lipid production using crude glycerol as a carbon source. Energy Procedia 9: 274–282.

Ponthein, W., Cheirsilp, B. (2011) Development of acetone butanol ethanol (ABE) production from palm pressed fiber by mixed culture of Clostridium sp. and Bacillus sp. Energy Procedia 9: 459–467.

Cheirsilp, B., Kitcha, S., Torpee, S. (2012) Co-culture of an oleaginous yeast Rhodotorula glutinis and a microalga Chlorella vulgaris for biomass and lipid production using pure and crude glycerol as a sole carbon source. Ann. Microbiol. 62(3) 987–993

Tran, H.T.M., Cheirsilp, B., Umsakul, K., Bourtoom, T. (2011) Response surface optimisation for acetone-butanol-ethanol production from cassava starch by co-culture of Clostridium butylicum and Bacillus subtilis. Maejo Int. J. Sci. Technol. 5(03): 374-389. (IF=0.171)

Majid, N., Cheirsilp, B. (2012) Optimal conditions for the production of monoacylglycerol from crude palm oil by an enzymatic glycerolysis reaction and recovery of carotenoids from the reaction product. Int. J. Food Sci. Technol. 47(4): 793-800. (IF=1.223)

Cheirsilp, B., Torpee, S. (2012) Enhanced growth and lipid production of microalgae under mixotrophic culture condition: Effect of light intensity, glucose concentration and fed-batch cultivation. Bioresour. Technol. 110: 510-516.

Kitcha, S. and Cheirsilp, B. (2013) Enhancing lipid production from crude glycerol by newly isolated oleaginous yeasts: Strain selection, process optimization and fed-batch strategy. Bioener. Res. 6(1): 300-310.

Louhasakul, Y. and Cheirsilp, B. (2013) Industrial wastes utilization for low cost production of raw material oil through microbial fermentation. Appl. Biochem. Biotechnol. 169(1): 110-122.

Charoenphun, N., Youravong, W., Cheirsilp, B. 2013. Determination of reaction kinetics of hydrolysis of tilapia (Oreochromis niloticus) protein for manipulating production of bioactive peptides with antioxidant activity, angiotensin-I-converting enzyme inhibitory activity and Ca-binding properties. Int. J. Food Sci. Technol. 48(2): 419-428

Charoenphun, N., Cheirsilp, B., Sirinupong, N., Youravong, W. 2013. Calcium-binding peptides derived from tilapia (Oreochromis niloticus) protein hydrolysate. Eur. Food Res. Technol. 236(1): 57-63

Louhasakul, Y. and Cheirsilp, B. (2013) Industrial wastes as a promising renewable source for production of microbial lipid and direct transesterification of the lipid into biodiesel. Bioresour. Technol. 142, 329–337

Komonkiat, I., Cheirsilp, B. (2013) Felled oil palm trunk as a renewable source for biobutanol production by Clostridium spp. Bioresour. Technol. 146: 200-207.

Loyarkat, S., Cheirsilp, B. and Umsakul, K. (2013) Direct conversion of sugars and organic acids to biobutanol by non-growing cells of Clostridium spp. incubated in a nitrogen-free medium. Appl. Biochem. Biotechnol. 171(7): 1726-1738.

Loyarkat, S., Cheirsilp, B. and Umsakul, K. (2013) Decanter cake waste as a renewable substrate for biobutanol production by Clostridium beijerinckii. Process Biochem. 48(12): 1933-1941.